J. Cancer Mol. 2: 37-43, 2006
Evaluation of Combination Use of Two
Radiosensitizers Iododeoxyuridine and Misonidazole: A
Biodistribution Study with Whole-Body Autoradiography
Jeng-Jong Hwang, Hsin-Ell Wang, Hsin-Yu Fang, Fu-Du Chen, and Frank
Q. H. Ngo
Department of Medical Radiation Technology and Institute of
Radiological Sciences, National Yang-Ming University, Taipei, Taiwan
To investigate the intratumoral localization and biodistribution of
the combination administration of two radiosensitizers,
and misonidazole, in the mouse model to evaluate the availability
and possible schedule for future clinical application.
Gamma-scintillation counting and whole-body autoradiography were
adopted to investigate the biodistribution of [131I]iododeoxyuridine
in KHT sarcoma-bearing C3H/HeN mice.
The amounts of
incorporated into the DNA of KHT sarcoma cells at 0.5, 2, 4, and
24-h postinjection with single dose were 3.76, 3.50, 3.41, and 3.29
mBq/cell, respectively. The tumor/brain ratios of
calculated from the % injected dose per gram tissue were 14.5, 13.0,
11.5, and 88.0, respectively, at 0.5, 2, 4, and 24 h after
injection; the tumor/blood ratios were 0.76, 0.76, 0.70, and 12.57;
while the tumor/muscle ratios were 3.22, 3.71, 2.56, and 22.00,
respectively. On the other hand, the tumor/brain ratios of
were 1.29, 1.21, 1.26, and 2.48 at 0.5, 1, 2, and 4 h after [18F]FMISO
injection, respectively; while the tumor/muscle ratios were 1.41,
1.30, 1.25, and 2.21. The tumor/muscle ratios were 4.0, 2.0, and
simultaneously for 4 h, and
[131I]IUdR 2 h followed by [18F]FMISO
for another 2 h, respectively. The whole-body
showed uptake of [131I]IUdR
peripheral region of tumor
at the deeper sites.
[131I]IUdR was accumulated at the superficial area while
[18F]FMISO at the deeper hypoxic site of tumor.
Combination use of both radiosensitizers, [131I]IUdR
first 2 h followed by [18F]FMISO
for another 2 h, may be
to enhance the radiosensitization during radiotherapy.
biodistribution; whole-body autoradiography;
radiosensitizer; [131I]IUdR; [18F]FMISO
9/7/05; Revised 12/8/05; Accepted 12/12/05.
Dr. Jeng-Jong Hwang, Institute of Radiological Sciences, National
Yang-Ming University, 155 Li-Nong Street Sec. 2, Taipei 112, Taiwan.
Phone: 886-2-28267064. Fax: 886-2-28201095. Email: firstname.lastname@example.org
IUdR, iododeoxyuridine; MISO, misonidazole; WBAR, whole-body
autoradiography; [18F]FMISO, [18F]misonida-zole;
%ID/g, % injected dose per gram tissue.